The Cell Wall-degrading Activities of Lysin Encoded by Chlorella Virus PBCV-1
小球藻病毒PBCV-1特异性溶壁酶(Lysin)的溶壁活性

Lysin was isolated from Chlorella NC64A lysate which was infected by Chlorella virus PBCV-1. The enzyme property detection of lysin shows that it contains at least three enzyme activities: chitinase, chitosanase and beta-1, 3-Glucanase. This is consistent with the compose of Chlolrella cell wall. Chitinase and chitosanase, especially chitinase plays a important role in the process of virus entry. One 52 kD chitinase, one 56 kD chitosanase and one 36 kD chitosanase were obtained after purified by FPLC.