PURIFICATION AND CHARACTERIZATION OF BANANA BUNCHY TOP VIRUS
香蕉束顶病毒的纯化及理化特性

Banana bunchy top virus(BBTV) was purified from banana plants (Fu Jian Province) with banana bunchy top disease by cycles of differential centrifugation and caesium sulphate density gradient centrifugation. These BBTV particles were isometric with a diameter of about 18nm, The A260 / A280 of purified preparations was about 1.30. A single coat protein of Mr 21kDa was identified with antibodies to BBTV particles from Australia by ECL-Western blotting. Nucleic acid extracted from BBTV preparations migrated as a discrete band of about 1.0kb. This band was sensitive to digestion by DNasel and Mung bean nuclease but not to RNaseA. The single-stranded virus-associated DNA of BBTV was characterized.