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OALib Journal期刊
ISSN: 2333-9721
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Screening and Identification of a Bacillus Strain Producing Butanol
产丁醇芽孢杆菌的分离、筛选与鉴定

Keywords: Butanol fermentation,Screening,Identification,Bacillus
丁醇发酵
,分离筛选,芽孢杆菌

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Abstract:

A facultative anaerobic butanol producing bacteria named C2 was isolated from soil planted with rehmanniae. C2 could produce 17.17 g/L total solvent (acetone, butanol and ethanol, ABE) in a 7% corn mash and the butanol yield reached 11.2 g/L amounting to 65.2% of the total solvent. Using corn straw hydrolysate as raw material (total sugar 25 g/L), 3.64 g/L total solvent was produced, among which 72.3% was butanol, reached to 2.63 g/L. According to the analysis of morphology, physiological and biochemcal characteristics and 16S rDNA sequence, this bacteria strain was identified as Bacillus sp., and was most related to B. vallismortis, B. Atrophaeus and B. mojavensis.

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