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Analysis and Identification of Pathogenic Bacteria by MALDI-TOF Mass Spectrometry
MALDI-TOF质谱技术分析与鉴定病原细菌研究

Keywords: MALDI-TOF-MS,Bacteria mass finger-printing,Identification,Pathogenic bacteria
MALDI-TOF
,MS,细菌质量指纹图谱,鉴定,病原细菌

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Abstract:

Based on Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI- TOF-MS) method for discrimination of pathogenic bacteria was developed. Established the standardization method for MALDI-TOF-MS analysis of bacteria and affecting elements of the analyzing result. The results obtained from twelve plants pathogenic bacteria of different genus, species and subspecies were analyzed by using MALDI-TOF showed that, MALDI-TOF-MS could be rapid and reliable identification of bacteria at the level of genus, species, and strains. Analytical process is simple, high sensitivity are advantages of this strategy.

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