The Variation of Genetic and Virulent of Xanthomonas oryzae pv. oryzae Strains and Mono-cell-clones from China, Japan and the Philippines
中国、日本和菲律宾水稻白叶枯病菌的母株与单细胞系的遗传与致病性差异的分析

The primers J3, IS1112, IS1113, and ERIC were used to analyze 17 tested groups (56 strains and their mono-cell-clones) of Xanthomonas oryzae pv. oryzae from China, Japan and the Philippines. The result showed: 1) The percentage were 52.9%(J3), 23.5%(IS1112), 29.4%(IS1113) and 35.3%(ERIC) which strains and their mono-cell-clones were in one patten of each primer, respectively. 2) Percentage were 29.4% which the similarity were over 90% between strains and their mono-cell-clones of 17 tested groups, while 52.9% of dissimilarity were 30%~41%; at level of 80% similarity, 9 tested groups which strains and their mono-cell clones were in one cluster(52.9% of 17 tested groups), these results suggested there were phylogenetic relationship between the strains. Also the differentiation were exits. Such as, Philippine strains, Pxo79 and Pxo112 were distributed into 3 clusters; the dissimilarity were 41% between strains and their mono-cell- clones of Pxo79, Pxo86, Pxo99 and Pxo112. These results indicated the strains were combined by different cells. 3) There was only 11.8% of tested strains which virulence of strains were equal to their mono-cell- clones in 17 tested groups. The result indicated that there were virulent difference between strains and their mono-cell-clones. No relationship were observed between UPGMA clusters and pathotypes.