Isolation, Selection of Microalgae for Lipid Production and Optimization of Its Nitrogen Resource and Carbon Resource in Autotrophic Culture
产油微藻的分离、筛选及自养培养氮源、碳源的优化

An autotrophic oleaginous Chlorella vulgaris with lipid productivity at 28.6 mg/(L?d), was isolated from the water sample of Dianchi Lake in Yunnan province. The effects of nitrogen sources, nitrogen concentrations, and inorganic carbon (NaHCO3) on cell growth as well as lipid accumulation of C. vulgaris were investigated. The results showed that the optimum nitrogen source and nitrogen concentration were sodium nitrate and 123 mg/L, respectively. Lipid content decreased with the increasing of nitrogen concentration. Biomass productivity and lipid productivity of C. vulgaris remarkably rose by addition of NaHCO3. The optimized concentration of NaHCO3 was 800 mg/L. Under the optimal conditions, biomass productivity and lipid productivity of C. vulgaris could reach up to 332.8 mg/(L?d) and 100.2 mg/(L?d), respectively, which were 3.6 and 3.4 times as much as the control group.