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微生物学通报 2011
Cell surface hydrophobicity of Ralstonia solanacearum
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Abstract:
Bacterial adhesion to hydrocarbons(BATH) and hydrophobic interaction chromatography(HIC) were employed to determine cell surface hydrophobicity(CSH) of Ralstonia solanacearum,respectively.The effects of the ratio of bacterial suspension to n-dodecane in the BATH assays and the injection volume of bacterial suspension in the HIC assays on the measurement of CSH were investigated.The optimal conditions for CSH analysis were as follow: the ratio of bacterial suspension(OD600=0.5) to n-dodecane was 2:1 and the ...