ISOLATION AND CULTIVATION OF THE ENDOPHYTE FRANKIA FROM THE NODULE OF ALNUS NEPALENSIS
蒙自桤木根瘤内生菌的分离培养

Four strains of Frankia were isolated from the nodules of Alnus nepalensis.Morphological study was made on them and the growth curve of Frankia sp.AnIZ was determined in vitro.The results shown that beside the medium and the isolating method,the growthstate of the host plant would also be an importent influence factor in the successfully isolatingFrankia from the nodules of Alnus nepalensis,"S"medium was a good medium for isolating andcultivating Frankl'a.Frankl'a grown slowly in vitro, it could attain its stationary phase in about 10days.