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Genetic Characterization and Antigenic Analysis of Hemagglutinin-neuraminidase Glycoprotein of Newcastle Disease Virus Isolates
新城疫分离毒HN蛋白的抗原性初步分析及分子特性研究

Keywords: Newcastle disease virus,Monoclonal antibody,Hemagglutinin-neuraminidase gene,Sequence analysis
新城疫病毒
,单克隆抗体,HN基因,序列分析,新城疫,分离毒,蛋白,抗原性,分析,分子特性,研究,Newcastle,Disease,Virus,Glycoprotein,Analysis,Characterization,亲缘关系,突变,发生,酸位,氨基,差异显著,显示,相关区域,受体结合

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Abstract:

Twenty Newcastle disease virus(NDV)strains were isolated from diseased chicken and geese in field outbreaks during 2005 and 2006 in some regions of Jiangsu and Guangxi,and the antigenic analysis of the all NDV isolates had been done based on the reaction spectrum with a panel of monoclonal antibodies to the HN glycoprotein.The entire ORFs encoding HN protein of these NDV isolates were amplified by RT-PCR successfully,cloned and sequenced.The resultant sequences of HN genes of 13 isolates of chicken origin a...

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