Progress in the Biodiversity of Nonculturable Microorganisms and Microbial Molecular Ecology
未培养微生物的研究与微生物分子生态学的发展*

Recent progress in molecular microbial ecology has revealed that traditional culturing methods fail to represent the scope of microbial diversity in nature, only a small proportion of viable microorganisms in a sample are recovered by culturing techniques. Molecular techniques, 16S rRNA sequencing, DNA-DNA hybridization, genetic fingerprinting technique and metagenomics etc, have become routine methods in microbial ecology, which are used to explore the diversity of uncultured microbial communities and obtain novel environmental DNA without any cultivation.