Optimization of Culture Condition for Novel Biopolymer Ss Production by Sphingomonas sp. Strain TP-3
鞘氨醇单胞菌TP-3合成新型生物聚合物Ss的发酵条件优化

Sphingomonas sp. TP-3 synthesizes a type of novel biopolymer Ss with properties of thickening, pseudoplastic, gelling and emulsification. Both single factor experiments and uniform design were employed in this study to optimize fermentation conditions of strain TP-3. The optimal fermentation paramenters in shaking flask were as follow: glucose 41.2 g/L, soybean meal 2.0 g/L, NaCl 0.85 g/L, K2HPO4 1.46 g/L, MgSO4 0.12 g/L, MnCl2 0.0075 g/L, FeSO4 0.002 g/L, initial pH 7.0, culture temperature 27°C, rotating speed 180 r/min. The yield of biopolymer Ss reached 21.5 g/L. As a low-cost biopolymer, Ss has wide prospect for application in oil recovery.