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Identification of a thermophilic bacterium and preliminary characterization of the secreted xylanase
一株产木聚糖酶嗜热菌的鉴定及酶学性质

Keywords: Thermophilic bacterium,Thermophilic xylanase,Zymogram analysis,Cellulase activity
嗜热菌
,嗜热木聚糖酶,酶谱分析,纤维素酶活性

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Abstract:

The bacterium isolated from a water sample of Yunnan tengchong hot spring can secrete a kind of thermophilic xylanase.It was identified and named as Geobacillus sp.PZH1 by morphologic observation,physio-biochemical characteristics and 16S rDNA sequence alignment.Subsequently,its secreted xylanase and the xylanase's characteristics were researched preliminarily.SDS-PAGE electrophoresis and zymogram analysis suggested that the xylanase's molecular mass was 69 kD;the optimum pH and temperature of the partially...

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