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cDNA'' CLONING AND SEQUENCING OF THE N-TERMINAL GENE OF BPI FROM CHINESE
中国人杀菌蛋白氨基端基因的克隆和序列分析

Keywords: BPI PT-PCR,cDNA cloninng,Sequencing
人杀菌蛋白
,PCR,cDNA克隆,序列分析

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Abstract:

The genes, named BG6o, BGnor and BGHGV, encoding human N - terminal ofbactericidal / Permeability increasing protein (BPI) were cloned by RP- PCR method usingmRNA templates isolated from HL - 6O cells and Peripheral blood lymphocytes of a normalPerson and a blood donor with hepatitis G virus(HGV) RNA. The results of DNA sequencingshowed that the BG60 had the same sequence as that reported, but both BGNor and BGHGV hadthree nucleotide substitutions, which resulted in one and two amino acid variatons respectively.The results suggested that N - terminal gene of BPI exists diversity.

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