PREPARATION OF DOUBLE MUTANTS THROUGH CROSSES BETWEEN COMPATIBLE AUXOTROPHIC MUTANTS IN HIGHER BASIDIOMYCETES
高等担子菌双重营养缺陷突变菌株的分离

In culer to get double auxotrophic muntants, two compatible auxotrophic mutants of (Pleurotus sajor-caju were mared with each other on CMA plates and also were done for Schizophyllum commmune. The fruit bodies were induced from those matings and the spores were collected from those produced fruit bodies, and then the monokaryons were isolated from those spores on CMA. Those monokaryons were subcultured onto the MMA, MMA one nutrient which was deficient for one parent and MMA another nutrient which was deficient for the other parent The monokareons that could not grow on these three kinds of media were suposed to be double mutants and this was further confirmed by subculturing them onto MMA supplemented by the two nutrients which were deficient for the two parents. A rather number of stable double auxotrophic mutants were selected and they are useful as a start strain in protoplast fusion study.