STUDIES ON QUANTITATIVE ANANLYSIS OF WHOLE-CELL SUGARSIN ACTINOMYCETES BY GAS CHROMATOGRAPHY-MASS SPECTRUM
气质联用定量分析放线菌全细胞单糖方法的研究*

Rapidly and accurately quantitative analysis of whole-cell sugars in some strains of actinomycetes by GC-MS was performed in this paper. After the polysaccharides were hydrolyzed, deoxidized and esterified , the alditol acetate derivates was analyzed by GC-MS. The derivates of rhamnose, ribose, arabinose , xylose, madurose, mannose, glucose, galactose were separated commendably under this condition. The ratio of the peak area was calculated to get the relative percentage of the various sugars.