A STUDY ON ULTRA LOW TEMPERATURE FREEZING OF ALKALOPHILIC MICROORGANISMS
嗜碱细菌的液氮超低温冻结保藏

In this paper, response of the 7 bacterial strains belonging to alkalophilic microorganisms subjected to liquid nitrogen freezing and thawing was reported. According to the cell survival rate, the protective efficiency of 10% glycerol and 5% DMSO was equal to the protective efficency of the same method used in general bacterial cultures after preservation at frozen state for 3 months. The cell survival rate of alkalophilic microoraganisms should be increased if using alkaline protective agent.