SCREENING OF STRAIN PRODUCING ALKALINE CELLULASE AND STUDES ON THE CHARACTERISTICS OF THE ENZYME SYNTHESIS
产碱性纤维素酶菌株的选育和酶合成基本特性

After combination treatment of Bacillus sp. x-6 with EMS and UV, a mutant EV23 was obtained from its vancomycine-resistant mutants. The alkaline cellulase activity was improved from 0.84u / ml to 3.53u / ml. The synthesis of alkaline GMCase by EV23 occurred almost conshtutively with distinct resistance to catabolite repression. When glucose was used as carbon source, the enzyme produchvity reached the highest at the concentration of 4%. IN addition, the enzyme synthesis that was related to the cell growth rate colsely proceeded synchronously with the bacterial growth. The presence of glucose and intermediated in TCA at higher levels caused repression of GMCase formation to some extent. The effects of exogenous ATP and cAMP on the enzyme production were also investigated.