PLASMID DETECTION FOR PURE CULTURES OF NODULAR ENDOPHYTES OF CORIARIA NEPALENSIS
尼泊尔马桑根瘤内生菌纯培养物的质粒检测

A rapid ingel lysis method was used to detect the plasmids among twenty one Frankia strains from Coriaria nepalensis and four confirmed Frankia strains from other host plants belonging to four genera of actinorhizal trees. Five isolates were detected harbouring plasmids which ranged from 13 to 20 kb in size. Both the Frankia strain Hr18 and the isolate Cs466 showed two plasmids, They appered to share the same plasbod pattern according to the size. Based on the number and size of the plasmids detected, twenty one Coriaria isolates were divided into four plasmid groups. The effect of bacterial growth and cell lysis conditions on plasmid detection was also explored.