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OALib Journal期刊
ISSN: 2333-9721
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EVALUATION ON DETECTION OF SHIGELLA CULTURES WITH THE DIAGNOSTIC TYPING PHAGE SET FOR ENTEROBACTERIACEAE
应用肠杆菌科诊断噬菌体检测志贺氏菌的评价

Keywords: Lytic patterns of phage,Enterobacteriaceae,Shigella,Enteroinvasive Escherichia coli
噬菌体裂解模式
,肠杆菌科,志贺氏菌,侵袭性大肠杆菌

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Abstract:

In this paper , 2280 strains of suspicious Shigella c culture were detected by the diagnostic typing phage set for Enterobacteriaceae, the routine identification of them were carried oat at the same time . The results showed that 100% of shigella cultures were lysed with 10~3RTD and 99.9% lysed with 1RTD of phage Sh. 12.3% of 65 non-shigeUa cultures agglutinating with typing serum of shigeUa was lysed with 10~3RTD, 4.6% lysed with 1RTD of phage Sh. The determination of lytic-pattern of 2215 shigella culture indicated that only 3 strains of Boyd 5 of lytic-pattern 3 were not reported in literature, the rest strains were consistent to fomer studies. The nonshigella cultures lysed by Sh 10~3RTD could be excluded with 1RTD and its lytic-pattern. 20 strains of Enteroinvasive Escherichia coli possessing shigeUa-related antigens could be differentiated by Sh 10~3RTD. 1RTD and its lytic-pattern.

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