Screeing of the High Lipid Production Strains and Studies on its Flask Culture Conditions
高产油脂酵母菌选育及摇瓶发酵条件的研究

A mutant of Lipomyces starkeyi was obtained through multiple mutagenesis (Ultraviolet and EMS) and named as L. starkeyi HL, which has the capability of accumulating substantial amount of lipid under N4indted culture conditions. Then seveal factors influencing the lipid accumulation were investigated and the optimum conditions of producing lipid were obtained: concentrations of waste sugar and the nitrogen source (NH4),SO4 were 165.7ml/L and 1.08g/L; C/N 61:1; initial pH5.0; inoculation volume 10%; temperature 28t; culture time 96h. This research analyzed the lipid from the mutant L starkeyi HL with MS/GC, it's content fatty acid were palmitic acid 33.2%, palmitoleic acid 3.4, steric acid 6.0%, oleic acid 50.8%, linoleic acid 3.55%, lindenic acid 1.53%, others 1.52%.