THE IDENTIFICATION OF MYCOBACTERIA BY PHOTOBIOTINMARK NUCLEI ACID PROBES
应用光敏生物素标记核酸探针鉴定分枝杆菌的研究

The DNA probes from separation strain of non-tuberculosis mycobacteria,and panchromosome of standard mycobaclerium spp. labelled by photo-sensitive biotin werehybridized with DNAs from known standard mycobacterium bovis (BCG strain)and variousnon-tuberculosis mycobacteria to identify mycobacteria strains by negative contrasl withEscherichia coli into species levcl rapidly with good specificity and accuracy. The chara-cteristics of this method include short-time consumed identification, ease operation, goodstability and noharm to human, and it is suitable to the identification of Mycobacleriumtubcrculosis and non-tuberculosis mycobacteria.