INFLUENCE ON PROPERTIES OF BACILLUS THURINGIENSIS YBT-1463 STRAIN BY CURING ITS RESIDENT PLASMIDS
消除内生质粒对苏云金芽胞杆菌YBT-1463特性的影响

This paper reports the influence on morphological, genetic, physiological and biochemical properties of Bacillus thuringiensis Wild-type stamen YBT-1463 by curing its resident plasmids. It showed that the plasmid-cured mutant BMB171 lost the ability to form the parasporal crystal, but its electro-rtransformation efficiency to 4 exogenous plasmids, pBMB121, pBMB304-lAb, pBMBLC and pBMB9748 was much higher than that of YBT-1463, and the highest transformation frequency could reach up to 4.8 x 10~4 fold, whereas there was no obvious influence on the sensitiveness of 10 antibiotics, the utilization of 19 carbon sources and 12 nitrigen sources materials as well as the growth properties between BMB171 and YBT-1463 was observed.