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微生物学通报 1999
PRESERVATION OF HALOPHILIC ARCHAEOBACTERIA
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Abstract:
Viability of the frozen cultures and freeze--dried cultures of 3 strains by using different concentrate of salt and different protectant was examined. The result clearly show that the protectant should contain swdium chloride for preservation of extreme halobacteria But it is not necessary that the salt concentration of protectant must be the same as it for growth. And 6% behalose as a Protectant for freeze-crying is more better than skimmed milk. Viability of the frozen and freeze-dried cultures of 77 strains after one year and 19 strains after two years was 100%.