Comparison between Effects of Traditional Chinese Medicine Treatment from the Lung and Treatment from the Intestine on the Level of VIP in Rats with Crohn''s Disease
从肺论治法和从肠论治法对克罗恩病大鼠肺与结肠VIP含量的影响比较

This study was aimed to observe effects of traditional Chinese medicine (TCM) treatment from the lung and treatment from the intestine on the level of VIP in rats with Crohn's disease. Rrat model of Crohn's disease was established by TNBS/ethanol enema. The model rats were treated experimentally from the lung (Huangqijiegeng Decoction) and from the intestine (Huangqihuanglian Decoction), respectively, by gastric perfusion. Changes in the level of VIP in rats with Crohn's disease were detected in zero, and three weeks after the treatment, respectively. The results showed that in zero week, the level of VIP in the lung of model group was decreased compared with that in normal control group, but there was no obvious difference. In the third week after treatment, the level of VIP in the lung of model group was significantly decreased compared with that in normal control group. And in different treatment groups the level of VIP in lung was significantly increased (P < 0.01),but there was obvious difference between Sulfasalazine group and normal control group (P < 0.05). In the zero and third week, the level of VIP in colon in model group was significantly increased compared with that in the normal control group (P < 0.01).In the third week, the level of VIP in colon in different treating group was significantly decreased compared with that in model group (P < 0.01). There was no obvious difference among the level of VIP in all treatment groups in the experiment. It was concluded that the imbalance of neuro-endocrineimmune network is the possible mechanism of lung injury in Crohn's disease, especially the imbalance expression of VIP. Both treatment from lung group (Huangqijiegeng Decoction) and from intestine group (Huangqihuanglian Decoction) can adjust the expression of VIP and relieve inflammatory reactions in the lung and colon. Treatment from the lung group (Huangqijiegeng Decoction) has an advantage in increasing the level of VIP in the lung, and improving the function of pulmonary vascular, adjusting local blood supply in lung tissues, and improving damage repair.