HPLC and UPLC Switch for TCM Analysis
中药复杂样品高效液相色谱和超高效液相色谱分析条件转换方法

Authors have developed a method to make a switch between HPLC and UPLC for analyzing a complex sample. In the study, water extracts of Radix Salvia miltiorrhizae was taken as a model sample. Retention parameters (a and c values) of 12 peaks were calculated on five linear gradients using both HPLC and UPLC. The relation ship for the a and c values on HPLC and UPLC was developed, respectively. with the linear equation, The a and c values on HPLC and UPLC Could be/ branferred each other, and predication and optimization was realized with calculated a and c values in the CSASS So ftware. We have obtained fine linear relationship for both a and c values under HPLC and UPLC. R2 for a and c linear equation resulted in 0.9914 and 0.9936 respectively. The a and c values of water- extracts of Ligusticum chuanxiong was calculated using UPLC, while UPLC based analysis was predicted with calculated a and c values. The UPLC chromatogram was similar to the simulated one. We realized the switch between HPLC and UPLC using the method, allowing compatible data for both techniques. The method is simple and accurate. It facilitates the predication and optimization of a complex sample