"Angling" method of affinity column array coupling with HPLC for the rapidly screening of bio-active components from TCMs
亲和阵列-色谱联用筛选中药活性成分新方法：钓鱼法

A novel "angling" method for the rapidly screening of bio-active component in Traditional Chinese Medicines (TCM) and natural products basing on the hyphenation of affinity column array with HPLC was proposed. The biotargets such as enzyme, receptor etc were used as fish baits on the fishhooks to "fish" the bio-active components from a complicated mixture library, i.e. TCMs and natural products. After affinity absorption, the high affinity components were transported to a hyphenated HPLC system to identify the chemical structures. The affinity array could provide the different target affinities of the components in the sample solution. Basing the method, a screening strategy for the angiotensin converting enzyme inhibitor from TCMs was presented.