Molecular Identification of Bungarus Multicinctus and Its Adulterants Based on COI Barcode Sequence
基于COI条形码序列的金钱白花蛇及其混伪品的DNA分子鉴定

The COI barcode sequences have been utilized to distinguish Bungarus parvus from its adulterants.The COI barcode sequences of 11 samples from 4 species of Bungarus parvus and its adulterants were amplified and sequenced.The intra-specific variation and the inter-specific divergence were calculated and the cluster dendrogram was constructed.The results showed that the intra-specific sequence variation of Bungarus multicinctus,the origin animal of Bungarus parvus,exhibited low and the maximum intra-specific K-2P distance was 0.0185.The inter-specific sequence divergence between B.multicinctus and its adulterants exhibited high.The minimum inter-specific K-2P distance was 0.1561.The cluster dendrogram constructed can be seen that the same genus is together,and the different species separated well.B.multicinctus and adulterants can be distinguished obviously by COI sequences.It is concluded that Bungarus parvus and its adulterants can be correctly identified by using COI barcode sequence.This study offered a new tool to identify Bangarus parvus from its adulterants.The COI region is potentially to be used in the identification of other animal species in traditional Chinese medicine.