Effect of Tongluo Jiunao Injection on the Expression of Semaphorin 3A in Middle Cerebral Artery Occlusion rats
Semaphorin 3A在局灶性脑缺血大鼠脑组织中的表达特征及通络救脑注射液的保护作用

This work observed the distribution of Semaphorin 3A(Sema 3A) in middle cerebral artery occlusion (MCAO) rats and the effect of Tongluo Jiunao injection (TLJN) on Sema 3A expression. Three groups were established, including the normal group, the control group, and the TLJN group. The expression of Sema 3A was observed by ELISA and double-immunofluorescent staining 12 h, 1 d, 3 d and 7 d after MCAO. The infarction volume was measured by TTC staining, and the level of GAP43 by ELISA. TLJN decreased the infarction volume and increased the expression of GAP43 compared with the control group. The expression of Sema 3A was increased after MCAO, and significantly decreased 3 d after TLJN injection. The results showed that the expression of Sema 3A may be related with the neuron injury after MCAO, and TLJN may protect the brain by inhibiting the expression of Sema 3A.