Application of Pulsed Field Gel Electrophoresis in the Herbal Genome Program (HerbGP)
脉冲场凝胶电泳技术在本草基因组计划中的应用

Pulsed Field Gel Electrophoresis(PFGE), which was developed in the 1980s, is mainly used to separate large linear DNA molecules (50 kb-12 Mb). With the development and implementation of the herbal genome program (HerbGP), PFGE is playing an increasingly critical role. In this study, we focus on the PFGE application in herbal genomic sequencing, including the measurement of the chromosome number and genome size of medicinal fungi, as well as construction of the genomic library and physical mapping. We also discuss the problems of how to use PFGE.