Extraction Optimization and Paeoniflorin Content Determination Method of Wen-Jing-Yang-Xue Mixture
温经养血合剂的提取工艺优选及芍药苷成分含量测定

The study was aimed to establish an optimized extraction method and paeoniflorin content determination method of Wen -Jing -Yang -Xue mixture. The extraction method of volatile oil was optimized by orthogonal experiment with different extraction rate of water amount, soaking and extraction time. The percolation extraction process was optimized by solvent volume and percolation rate. And the content of paeoniflorin was determined by HPLC method. The results showed that the optimal extraction method of volatile oil was to soak for 0.5 h with 6 times water and then extract for 8 h. The optimal extraction technology was to soak for 24 h with 8 times of 70% ethanol. And the speed for percolation extraction is 5mL/min?kg.