Effect of Glytan on Liver Fibrosis in Portal Hypertensive Rats
肝络通对门静脉高压症大鼠肝组织纤维化的影响

This research was aimed to observe the effect of Glytan on rat liver fibrosis and investigate the mechanism of portal hypertension (PHT). SD male rats with the weight between 240 g to 260 g, were randomly divided into sham group, model group, propranolol group, and Glytan group according to weight. PHT and liver fibrosis were induced by common bile duct ligation. After 2 and 4 weeks, hydroxyproline (Hyp) content of liver tissues was tested by spectrophotometer. The collagen formation was evaluated by Masson staining and ultrastructural changes by transmission electron microscopy (TEM). The results showed that Glytan inhibited Hyp production at 2 and 4 weeks. Masson staining revealed the same results with Hyp. Glytan inhibited pseudolobule formation. TEM showed swelling sinusoid endothelial cells (SEC), reduced fenestra between SEC, widened Disse space, reduced hepatocyte microvilli in model group rats. At 4 weeks, loss of hepatocyte microvilli and basement membrane were clearly seen. Glytan promoted degeneration of matrix and restoration of SEC at 2 and 4 weeks. It was concluded that Glytan inhibited collagen formation and ameliorated sinusoidal capillarization. This is one of the mechanisms of Glytan decreases portal venous pressure. The results provided precise and reliable proof to develop new treatment for PHT.