Characteristics of G Protein Signaling Pathway and Related Gene Expression in Thyroid of Mice with Different Qi-Deficiency Syndromes
不同气虚证小鼠甲状腺G蛋白通路及相关基因转录特征

This study aimed to analyze characteristics of G protein signaling pathway and related gene expression in thyroid of mice with different qi-deficiency syndromes(QDS).Quantitative four diagnosis,syndrome differentiation methods,and Affymetrix GeneChip Mouse Exon 1.0 ST Array were used.Thyroid gene expression of normal mice and tumor mice with QDS were detected.Genes of G protein signaling pathway and related hormones in gene chips were selected.And then,characteristics of gene expression were analyzed.The results showed that the expression of thyroid stimulating hormone(TSH) and TSHR were increased in thyroid of QDS mice.And the adenylate cyclase(AC) and protein kinase A(PKA) in G protein signaling pathway were increased in thyroid of QDS tumor mice,but not in the normal QDS mice.The Ras genes also follow the same trend.It is concluded that most inactive genes may be the material base of QDS among normal mice.However,QDS among tumor mice was different from normal QDS mice.Since the QDS in tumor mice is caused by excess,and the thyroid was in compensation condition.