Establishment of Disease-syndrome Combination Rat Model of Rheumatoid Arthritis with Spleen Deficiency
类风湿性关节炎脾虚证病证结合动物模型的建立

This study was aimed to establish a disease-syndrome combination rat model of rheumatoid arthritis(RA) with spleen deficiency.A group of 60 six-week-old Sprague-Dawley rats were randomly divided into normal control group and reserpine(RSP) group.Spleen deficiency model rats groups were made by intraperitoneal injection of RSP.Then rats of RSP groups were subcutaneously injected with collagen protein to induce CIA.The incidence of the model was observed and the mechanism was analyzed by enzyme-linked immunosorbent assay(ELISA).The results showed that compared with the CIA normal group,the degree of joint swelling and joint damage was significantly increased in the RSP CIA model.The RSP CIA model rats were noted with spleen deficiency syndrome similar to clinical symptoms,such as appeared apathetic,squinting,sparse dry dull coat,bowed back,swollen ankle,fatigue,lazy move,get together,eating less,weight loss and loose stool.Compared with the CIA control rats,spleen deficiency impaired the function of intestinal mucosa by disordering the intestinal villi,covering large amount of goblet cells in epithelium,and inducing exfoliation,degeneration and necrosis fluff pieces.The content of anti-type Ⅱ collagen antibody,IL-6,IL-10 and IFN-γ was increased.It was concluded that the rat model of RA disease-syndrome combination with spleen deficiency has been established.Spleen deficiency plays a vital role in RA.