Determination of Berberine in Taxilli Herba and Its Host Plants -Clausena lansium by RP-HPLC
RP-HPLC测定桑寄生及其寄主植物黄皮树盐酸小檗碱的含量

This study was aimed to determine contents of berberine in Taxilli Herba and its host plants Clausena lansium,and further to explore the effect of host plants on Taxilli Herba.Berberine was extracted with CH3OH-HCL(100:1) solution by ultrasound.The content of berberine was determined by RP-HPLC.Agilent C18 column(250 mm × 4.6 mm,5 μm) was used for the determination,with a flow rate of 1.0 mL·min-1 and column temperature of 25℃.The mobile phase were acetonitrile-warter-phosphoric acid-triethylamine(72:28:0.01:0.05).The detection wave length was 284 nm.The results showed that contents of berberine in Taxilli Herba and its host plants-Clausena lansium were 0.11 mg·g-1-1.35 mg·g-1 and 0.14 mg·g-1-7.58 mg·g-1,respectively.The highest content of berberine in Taxilli Herba is as high as 132.10% as that in Clausena lansium.It was concluded that host-plants exert a very important effect on the quality of Taxilli Herba.With different host plants,the content chemical composition in Taxilli Herba were different.