Effect of Zhichan Decoction on Neural Stem Cells Differentiation in Parkinson''s Disease Model Rats
止颤汤对移植入帕金森病大鼠脑内神经干细胞增殖分化作用的研究

This study aimed to discuss the effect of Zhichan decoction on neural stem cell differentiation in Parkinson's disease (PD) model rats. PD model rats were divided into four groups. In the normal group and model group, 0.9% normal saline was administered intragastrically. Normal culture medium was transplanted in the brain. Zhichan decoction was administered intragastrically in the treatment group after neural stem cell transplantation. TH protein was checked with immunohistochemical method and mRNA expression of Ptx3, Nurrl, Shh were checked with Rt-PCR from brain slices on 7d, 14d and 28d, respectively. The results showed that compared with control group, TH protein was significantly increased in the treatment group on 7d and 14d. In the brain, mRNA expression of Ptx3 and Shh was significantly increased at some time points. It is concluded that Zhichan decoction has the effect of promoting neuron stem cell differentiation in PD model rats.