Cytoprotection of 13 Pakistan Medicinal Plants on Neurotoxicity of PC12 Cells
13种巴基斯坦草药对PC12细胞保护作用的研究

This study aimed to investigate the cytoprotection of 20 extracts from 13 Pakistan medicinal plants on PC12 cells injured by H2O2 and glutamate (GLU). Different concentrations of H2O2 and GLU were used to establish the toxicity models on PC12 cells. Cell viability was determined by MTT assay. The results showed that the extracts of Mucuna pruriens L and Terminalia chebula had significantly proliferative effects on PC12 cells (P <0.05).Additionally, extracts of Lawsonia alba, Opuntia dillenii Haw., Celastrus paniculata, Terminalia chebula showed markedly cytoprotection against H2O2-induced cell death (P<0.05). Similar results were observed in GLU injured model, which showed that extracts of Lawsonia alba, Opuntia dillenii Haw., Mucuna pruriens L. and Terminalia chebula attenuated GLU-induced toxicity obviously (P<0.05). Among all extracts, only Terminalia chebula had cytoprotection against both H2O2 and GLU induced neurotoxicity with proliferative effect on PC12 cells. It isconcluded that extracts of Terminalia chebula, Ocimum basilicum, Mucuna pruriens L. have promoting effect on the proliferation of PC12 cells. Terminalia chebula, Lawsonia alba, Opuntia dillenii Haw., Asparagus officinalis,Celastrus paniculata, Ocimum basilicum extracts possess significant antioxidative activities. And the extracts fromMucuna pruriens L, Terminalia chebula, Lawsonia alba, Opuntia dillenii Haw., Asparagus officinalis, Glycyrrhiza glabra L. can decrease damages induced by GLU in PC12 cells. The results suggested that Terminalia chebula hadbetter cytoprotection against injured models on PC12 cells. Results of this study provide a preliminary experimental basis of screening and developing new agents for neurodegenerative diseases.