Studies on Bioequivalence of Activity based on “Spectrum-Effect Integrated Fingerprint” and Activity In Vivo
基于谱效整合指纹的体外活性与体内活性的生物等效性研究

This work was aimed to study the bioequivalence of activity based on "spectrum-effect integrated fingerprint" and activity in vivo.The hydrogen peroxide(H2O2) scavenging activity fingerprints of 9 batches of Crataegus leaves collected from different provinces were established through an on-line high-performance liquid chromatography-diode array detector combined with chemiluminescence detection(HPLC-DAD-CL) system.As the positive control,if resveratrol(1 μg) was presumed as a potency unit,the relative activities of active peaks were calculated as potency unit according to standard potency curves of resveratrol.And the relative total activities of Crataegus leaves should be the sum of potency of all characteristic peaks.A total of 130 mice were randomly divided into 13 groups,the acute ischemic anoxic myocardial injury was induced by subcutaneous injection isoproterenol in mice,the survival time in mice with hypoxic state was recorded and the life extension rate of the treated group was studied.The correlation between the H2O2 scavenging capacity of Crataegus leaves based on HPLC-DAD-CL and the protective effect in mice with myocardial hypoxia was investigated.And the content of total flavonoids was determined to make the results above more authentic.It is found the antioxidant activities in vitro and in vivo both confirm with each other,which means the on-line activity screening method and antioxidant-activity integrated fingerprint research platform can be further promoted.