Molecular Identification of Medicinal Plants in Aspidistra
蜘蛛抱蛋属药用植物分子鉴定研究

To test the DNA molecular markers(rbcL,matK,psbA-trnH,psbK-psbI,nr ITS and ITS2) to identify medicinal plants in Aspidistra,the efficiency of PCR amplification and sequencing,intraspecific variation and interspecific divergence and identification efficiency were used to evaluate these loci.The results showed that through analysis of 20 samples of 11 species of Aspidistra,rbcL、matK、psbA-trnH and psbK-psbI had high efficiency of PCR amplification and sequencing and the success identification rate of matK was the highest(85%) in single sequence.The discrimination ability of different sequences was shown higher using the sequence combinations,in which the success identification rate of matK+psbK-psbI was 100%.Therefore,matK+psbK-psbI could be used to identify the medicinal plants in Aspidistra.