Effects of Fas\Fasl,P53 Protein Expression in Retinal Ischemia-Reperfusion Injury Treated by Mongolian Medicine Zhen-Bao Pills
蒙药珍宝丸对视网膜缺血再灌注损伤Fas\Fasl、P53蛋白表达的影响及意义

The study was aimed to explore basic protective effects of retinal ischemia-reperfusion injury and effects on the expression of Fas,Fasl,P53 and apoptosis occured in retinal ischemia-reperfusion injury of rabbit model.Ninety-five healthy rabbits without eye disorders were selected.They were randomly divided into four groups,which are normal control group,ischemia-reperfusion group,Zhen-Bao Pills group,and VE group.According to time after reperfusion,ischemia-reperfusion group,Zhen-Bao Pills group and VE group were further divided in to 1 h,6 h,12 h,24 h,48 h and 72 h subgroup.Rabbit model of experimental retinal ischemia-reperfusion injury was made by increasing intraocular pressure.Then,the sample was processed with HE staining and immunohistochemical(SABC) staining.Histological and ultrastructural changes in retina were observed.Results of SABC staining were analyzed by compute picture analytic system.The result showed no Fas positive cells in the normal retina.Fas expression was gradually increased at 6 h.It reached a peak at 24 h.And then,it decreased at 48 h.Similarly,Fasl expression was at peak from 24 h to 48 h in GCL and INL of retina.And the peak of P53 expression was at 24 h.Then it was decreased gradually at 72 h.Zhen-Bao Pills administered before reperfusion ameliorated the retinal tissue damage.It also diminished Fas,Fasl and P53 expression in retinal ischemia-reperfusion remarkably.Fas expression was obviously decreased at 6 h,12 h,24 h,and 48 h in treatment group.Zhen-Bao Pills decreased Fasl expression at 12 h,24 h,48 h.P53 expression was decreased at 6 h,12 h,24 h,48 h.It is concluded that retinal ischemia-reperfusion injury can cause increasing of Fas\Fasl and P53 protein in retina.Zhen-Bao Pills can inhibit the increasing of Fas\Fasl and P53 protein in retinal ischemia-reperfusion injury.Zhen-Bao Pills have protective effects against retinal ischemia-reperfusion injury.It can be used in the clinical treatment of ischemia-reperfusion injury.