Comparison between Rheum palmatum L. and Rheum franzenbachii Munt. on Pharmacological Effect and Toxicity
正品及伪品大黄药效与毒性的比较研究

This study was aimed to verify disadvantages of the mixture of clinical using of Rheum palmatum L.(RPL) and Rheum franzenbachii Munt. (RFM) by comparison of their pharmacological effect and toxicity. In the in vivo experiment, potential time of defecation and quantity of feces were employed, the promotion of small intestine was observed to evaluate herbs on the intestinal function. The clotting time was employed for blood coagulation system. The lethal half dose and maximum tolerant dosage were carried out for the acute toxicity. In vitro experienment, the cytotoxicity on HepG2 cell line by MTT assay was employed for the toxicity. The results showed that there is a distinct promotion of feces after RPL administration, while no effect appeared after RFM administration. For the promotion on small intestine, RFM had a positive effect, but RPL had a negative effect. The large dosage of RPL promoted the clotting time, while RFM had no effect. In toxicity, RFM showed heavier than RPL in vivo and in vitro. Rhapontin, the major component of EFM, showed negative toxicity by acute toxicity and cytotoxicity essay. It was concluded that there are apparent differences between both herbs in pharmacological effect and toxicity, so they are not suggested the use in mixture or substitute in clinic.