Hepatotoxic Effect and Lipid Oxidative Damage of Diethylhexyl Phthalate (DEHP) on Mice
邻苯二甲酸二乙基己酯(DEHP)对小白鼠肝脏毒性及脂质过氧化损伤

To study the liver toxicity mechanism and lipid peroxidation induced by diethylhexyl phthalate (DEHP) on mice, eighty Kumming mice were divided into 4 groups randomly, which male was half. The animals were exposed to DEHP for 4 weeks. Then changes in liver and weight of the mice were detected. In the meantime, the activities of GPX and SOD, the contents of H2O2 and MDA in the blood and liver tissue were measured. The results of ANOVA test show that the weight of mice decreased significantly with increase of DEHP (p<0.01). The coefficient of liver/body with high dose group increased significantly. There were evident liver tissue damages in high dose group by HE staining. GPX activities of DEHP exposure groups in the blood tissue (except the 75 mg·kg-1 group) and liver tissue, the contents of H2O2, the contents of MDA and the activities of SOD in the liver tissue (except the 75 mg·kg-1 group) were significantly different(p<0.05,p<0.01) from the control group. Therefore, it was deduced that the liver toxicity mechanism of DEHP to mice was the lipid peroxidation.