Oxidative Damage Induced by Formaldehyde and Benzene Series Inhalation on Mice Lung
甲醛及苯系物混合暴露对小鼠肺脏的氧化损伤作用

To study the oxidative damage on mice lung induced by acute inhalation of formaldehyde,benzene,toluene and xylene, 50 Kunming male mice were randomly divided into one control group and four exposure groups. Concentrations of formaldehyde,benzene,toluene and xylene of exposure group 1 to 4 were: 1.0 +1.1 +2.0 +2.0 μg·L-1, 3.0 +3.3 +6.0 +6.0 μg·L-1, 5.0 +5.5 +10.0 +10.0 μg·L-1, 10.0 +11.0 +20.0 +20.0 μg·L-1, respectively,which corresponded to 10,30,50,100 times of indoor air quality standard (GB/T 18883|2002). Mice were exposed to mixture of VOCs in a static cabinet for 10 days, which exposed VOCs for 2 hours per day, and then oxidative damage markers were measured. The results show that the increase of weight of mice exposed to VOCs was lower than that of the control group. The liver and spleen coefficient of mice in exposure groups were significantly lower than that of the control group. Exposure dose rising, contents of ROS and MDA increased, activity of T|AOC, GSH, CAT, GSH|Px and SOD decreased. ROS and MDA contents were significantly positively correlated to, while GSH content was negatively correlated to it. Thus, it was deduced that acute inhalation of formaldehyde, benzene, toluene and xylene cause oxidative damage on mice lung. Moreover ,the joint toxic effect is stronger than single gas. ROS, MDA and GSH can be used as oxidative damage sensitive biological markers to evaluate acute toxicity of VOCs .