Structure of the Mitochondrial Control Region Sequence of Hapalogenys nitens
斜带髭鲷线粒体DNA控制区序列的结构分析

Complete sequences of mitochondrial DNA (mtDNA) control region from total 50 samples of cultured and wild Hapalogenys nitens of South China Sea were amplified by Polymerase Chain Reaction (PCR), the length ranged from 788-790bp. All sequences were aligned by Clustal X with other Perciformes species from GenBank and the structures of the control region were analyzed. Three domains of the control region, the termination associated sequence domain (TAS), the central conserved domain (CD) and the conserved sequence block domain (CSB) were identified from the aligned sequences. A termination associated sequence (TACAT) and its reverse complementary sequence (ATGTA) were found in the TAS domain. Three conserved blocks (CSB-F, CSB-E, and CSB-D) in the CD domain and three conserved sequence blocks (CSB-1, CSB-2, CSB-3) in the CSB domain were also identified in the control region of H. nitens. In addition, molecular phylogenetic tree based on complete D-loop sequences was constructed by Bayesian method. The result showed that, some samples from the cultured and wild H. nitens were grouped in small branches, respectively. But all samples between cultured and wild H. nitens were clustered together in the whole tree. In this study, the genetic difference between cultured and wild H. nitens captured in this sea was not so significant.