ANESTHETIC METHOD OF GOBIOCYPRIS RARUS
稀有鮈鲫麻醉方法研究

As a new experimental fish, Gobiocypris rarus has been wildly used in scientific studies in China since 1990. In this study, narcosis effects of MS-222, Urethan and Benzocaine on Gobiocypris rarus were observed to provide anaesthetic method for the laboratory animal. The results showed that MS-222 and Benzocaine were effective to rapidly anaesthetize for Gobiocypris rarus, whereas Urethan was disable. Sequential effects on behavior were observed when the fish was exposed to different concentrations of anesthetics for different time. Based on the behaviors, low-grade sedation, deep sedation, low-grade anesthesia, medium anesthesia, deep anesthesia and medullary anesthesia stages were determined. Considering the effect time, recovery time, maintain time and survival rate, 60 mg/L of Benzocaine and 100-110 mg/L of MS-222 were suggested as suitable dosage for deep anesthesia.