Isolation and Molecular Identification of Endophytic Bacteria from Lateolabrax japonicus Net Cage Culture in the Wujiang River
乌江网箱养殖患病花鲈的细菌分离与分子鉴定

Abstract:The present experiment was undertaken with the tranditional microbiology method to isolate the endophytic bacteria from Lateolabrax japonicus of net cage culture in wujiang river and 9 strains were isolated.The 600bp fragments of 16S rRNA gene of nine strains were amplificated by PCR methold. These 600bp sequences were successfully sequenced and were entered into the NCBI (http://www.ncbi.nlm.nih.gov/).Objective to analyse the homology by BLAST tool. It shows that nine stains are Aeromonas salmonicida (homology of 98%), Acinetobacter (homology of 97%), Klebsiella pneumoniae (homology of 98%), Aeromonas hydrophila (homology of 99%), Shewanella baltica (homology of 99%), Staphylococcus saprophyticus (homology of 99%), Bacillus subtilis (homology of 99%), Serratia proteamaculans(homology of 95%) and Aeromonas salmonicida (homology of 97%) respectively.