Effects of Formaldehyde on Activity of Aldose Reductase in Rat Lung Cells and AR Function Study
甲醛对大鼠肺细胞醛糖还原酶的影响及醛糖还原酶功能研究

In order to study effects of formaldehyde (FA) on activity of aldose reductase (AR) in rat lung cells and function of AR in lung cell injury, Firstly, effects of FA on activity of AR in rat lung cells and inhibition of berberine hydrochloride on AR activity were observed by rat lung cell detachment and culture. Secondly effects of FA and berberine hydrochloride on cell cycle and apoptosis of rat lung were detected by flow cytometry. The results showed that AR activity was increased significantly in rat lung cells that were exposed to 1.0mmol·L-1 FA for 24h; Berberine hydrochloride displayed inhibition on AR activity; FA could induce apoptosis of rat lung cells, decrease of cell percent of G0/G1 and S phase and increase of cell percent of G2/M phase; The percent of apoptosis in rat lung cells that were exposed to FA and berberine hydrochloride were higher than that in rat lung cells exposed to FA only. Meanwhile, cell percent of G0/G1 phase was increased, and cell percents of S and G2/M phase were decreased. It can be concluded that FA could cause increase of AR activity in rat lung cells; and the increase of AR activity could reduce apoptosis, thus it had protection on rat lung cell damage induced by FA.