Hemolytic Activity Studies of Several Harmful Alga Strains
4种典型有害赤潮原因种的溶血特性研究

The hemolytic activities of four harmful red tide organisms were investigated. The harmful algae included two strains of Heterosigma akashiwo, Alexandrium minutum, Prorocentrum donghaiense and Karlodinum micrum. The hemolytic activities on crucian erythrocytes for the cell free culture supernatants(CFCS)and ultrasonic ruptured cell suspensions (URCS)were measured respectively. The results showed that: the hemolytic activities of the red tide organisms were species-specific, and different between the CFCS and URCS. Heterosigma akashiwo had the most significant hemolytic activity, the hemolytic activity in the CFCS(60~100%)was much greater than that in the URCS(30~60%);Karlodinum micrum had obvious hemolytic activity, the hemolytic activity of the URCS(70%)was higher than that of the CFCS(30%);Both Prorocentrum donghaiense and Alexandrium minutum had relatively lower hemolytic activities, the hemolytic activity of Alexandrium minutum was mainly in the CFCS(CFCS: 30%, URCS: 10%). The difference was not significant in two parts of Prorocentrum donghaiense. The hemolytic activity characteristics of the different species and different situation reflect some mechanisms of the organism's harmful effects.