Microplate Luminometry for Toxicity Bioassay of Environmental Pollutant on a New Type of Fresh Water Luminescent Bacterium (Vibrio-qinghaiensis sp.-Q67 )
测定环境污染物对青海弧菌发光强度抑制的微板发光法研究

A new microplate luminometry for the toxicity bioassay of environmental pollutant on one of fresh water luminescent bacteriums,Vibrio-qinghaiensis sp. -Q67,was developed using VeritasTM Microplate Luminometer to measure the luminous intensity of Q67. Effects of pH,bacterial density and reaction time on the bioassay were systematically investigated. The method was successfully employed in the toxic effect test of 7 phenols on Q67. Using non-linear iterative least square technique,the dose-response curves(DRC) of all phenols and heavy metals were accurately fitted with the correlation coefficients between the fitted and observed responses being greater than 0.99. The median effective concentration (EC50) of all phenols and metals were accurately measured from the DRC models. Compared with some literatures,this bioassay is a fast easy-operate and cost-effective method with high accuracy.