The Research of Aldicarb and It''s Toxic Metabolites on DNA Potential Harm
涕灭威及其有毒代谢产物对DNA潜在损伤研究

Effects of Aldicarb and it's toxic metabolites,on the rates of micronucleus formation in root tip cells of Vicia faba and Allium sativum, and on the adduct actions to calf thymus DNA, respectively were studied in the present investigation. In some concentrations, micronucleus formation in root tip cells of Vicia faba and Allium sativum were induced by aldicarb, aldicarb sulfone and aldicarb sulfoxide. And with the increase of concentrations of aldicarb, aldicarb sulfone and aldicarb sulfoxide, the rates of micronucleus formation rosed in root tip cells. Compared with negative control group, results showed that in high concentration, rates of micronucleus formation in root tip cells of Vicia faba and Allium sativum were remarkably different (p<0.05), and the effects of aldicarb sulfoxide was more obvious than the effects of the others. The results indicated that aldicarb, aldicarb sulfone and aldicarb sulfoxide may exhibited carcinogenicity in root tip cells of Vicia faba and Allium sativum. And the distinct test of the partnership data difference showed that the sensitivity of micronucleus experiment in root tip cells of Vicia faba was obviously higher than that of Allium sativum. Using UV spectrum, the effects of Aldicarb and it's toxic metabolites on DNA were studied,the positions and intensions of absorbing peaks were changed, which implied aldicarb, aldicarb sulfone and aldicarb sulfoxide could bind to ctDNA to produce DNA adduct. Aldicarb and it's metabolites might result in the gene mutation and bring trauma to the DNA of the organisms through producing DNA adducts.