A Study of Single Cell Gel Electrophoresis for Detecting the DNA Damage and Repairing Caused by Acrylamide of Two Kinds of Cells in Mice
利用彗星实验检测丙烯酰胺对小鼠两种细胞的DNA损伤和修复

The DNA damage of two kinds of cells induced by acrylamide (AA) in mice were investigated using single cell gel electrophoresis, and the sensitivity to AA between reproductive cells and peripheral blood lymphocytes was compared. After kunming male mice were exposed to AA by ip at the concentrations of 50mg·kg-1(bw), the DNA damage of reproductive cells and peripheral blood lymphocytes from the mice were analyzed using single cell gel electrophoresis on the 1, 2, 4, 6, 8, 10, 12d. The results showed that significant comets of reproductive cells and peripheral blood lymphocytes (p<0.05) were induced by acrylamide at different sampling time, compared with the control group. The tail length of DNA migration of the two kinds of cells decreased as time extended, and acrylamide induced more serious DNA damage in reproductive cells than in peripheral blood lymphocytes (p<0.05). AA could induce DNA damage both in reproductive cells and peripheral blood lymphocytes. The DNA damage caused by acrylamide could be repaired to a certain degree. Acrylamide could induce more serious DNA damage in spermary tissue than in peripheral blood lymphocytes.